Paper 4 · November 2004 · Enzymes
In a Benedict's test, five glucose standards from 0.001 to 0.005 mol/dm3 are each mixed with Benedict's solution and heated in a boiling water bath for 5 minutes. Which colour is expected for the HIGHEST standard, 0.005 mol/dm3?
ALight yellow
BLight orange
CDark orange
DGreen
Explanation
The Benedict's colour scale runs from green at the lowest reducing sugar concentration to a dark orange precipitate at the highest; the 0.005 mol/dm3 standard, the most concentrated, gives dark orange.
Derived from ZIMSEC Biology 9190/4 Paper 4, November 2004, Q1