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Paper 2 · Genetic Engineering

Name the class of enzyme used in genetic engineering to cut a DNA molecule at a specific base sequence.

Model answer

restriction enzyme

Also accepted: restriction endonuclease, restriction enzymes, restriction endonucleases

Explanation: These enzymes recognise a short palindromic sequence and cut both strands there, so the same fragment is released every time. That reliability is what lets the same gene be cut out of a chromosome again and again.

Derived from ZIMSEC Biology Paper 2, Nov 2004, Q3

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