A recombinant plasmid carrying an antibiotic resistance gene is used to transform bacterial cells. Which of the following is a standard method for identifying which bacterial colonies have successfully taken up the recombinant plasmid?

Culturing the bacteria on a medium containing the antibiotic to which the plasmid's marker gene confers resistance; only bacteria that took up the plasmid will survive and grow.

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A Level Biology

Genetic Engineering Questions and Answers

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Question 1 of 4genetic engineering / recombinant DNA technology

In genetic engineering, a plasmid and a human gene are cut with the same restriction enzyme so that their cut ends are complementary. Which enzyme is then used to join (splice) the human gene into the cut plasmid to form a recombinant plasmid?

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Paper 2genetic engineering / recombinant DNA technology
In genetic engineering, a plasmid and a human gene are cut with the same restriction enzyme so that their cut ends are complementary. Which…
Paper 2genetic engineering / recombinant DNA technology
A recombinant plasmid carrying an antibiotic resistance gene is used to transform bacterial cells. Which of the following is a standard…
Paper 2recombinant DNA technology
During the formation of recombinant DNA, an mRNA molecule for the required gene is isolated and an enzyme is used to synthesise a…
Paper 2recombinant DNA technology
In recombinant DNA technology, both the DNA containing the required gene and the vector (e.g. plasmid) DNA must be cut at specific…

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Genetic Engineering Questions and Answers

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In genetic engineering, a plasmid and a human gene are cut with the same restriction enzyme so that their cut ends are complementary. Which enzyme is then used to join (splice) the human gene into the cut plasmid to form a recombinant plasmid?

A recombinant plasmid carrying an antibiotic resistance gene is used to transform bacterial cells. Which of the following is a standard method for identifying which bacterial colonies have successfully taken up the recombinant plasmid?

During the formation of recombinant DNA, an mRNA molecule for the required gene is isolated and an enzyme is used to synthesise a complementary DNA (cDNA) copy of it. Which enzyme carries out this conversion of mRNA into cDNA?

In recombinant DNA technology, both the DNA containing the required gene and the vector (e.g. plasmid) DNA must be cut at specific recognition sequences into fragments before the gene can be inserted into the vector. Which type of enzyme cuts DNA in this way?

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